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Diversité génétique des souches de Listeria monocytogenes isolées dans la filière porcine en France / Listeria monocytogenes genetic diversity in the pig and pork sector in France

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Les Cahiers de l'IFIP, 6(1), 1-7 - La revue R&D de la filière porcine française

Listeria monocytogenes est une bactérie pathogène ubiquitaire, transmissible à l’homme via la consommation d’aliments contaminés. Elle peut être isolée d’une grande variété d’aliments, en particulier les aliments à base de viande de porc. Entre 2015 et 2017, l’Ifip et l’unité SEL (Salmonella-Listeria) du Laboratoire de Sécurité des Aliments de l’Anses de Maisons-Alfort ont mené une étude qui visait à obtenir une meilleure connaissance de la diversité des souches de Listeria monocytogenes (Lm) afin de mieux caractériser la manière dont elle circule dans la filière porcine. Vingt-six complexes clonaux (CCs) majeurs ont été identifiés dans la filière porcine. Les CC37, CC77 et CC59 étaient associés au compartiment élevage mais pas aux compartiments environnement de production ni produits finis. Les CC121 et CC9 étaient fortement associés au maillon transformation de la viande de porc. Le CC121 n’a pas été retrouvé parmi les souches isolées du compartiment élevage. Le CC9 était associé aux produits finis, et en particulier la viande de porc non transformée, mais ni à l’élevage, ni à l’environnement de production. Aucun des CCs identifiés dans ce projet n’était spécifique de la filière porcine c’est-à-dire qu’ils étaient également identifiés dans les autres filières de production alimentaire. Les complexes clonaux CC1, CC2, CC6 identifiés en filière porcine ont été décrits comme hyper virulents chez l’homme par Maury et collègues en 2016.

35,00 €
2020

Population genetic structure of Listeria monocytogenes strains isolated from the pig and pork meat production chain in France

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Benjamin Félix (Anses) et al., Food Micro, 3-6 septembre 2018, Berlin, Allemagne

Listeria monocytogenes is an ubiquitous pathogenic bacterium, transmissible to humans through the consumption of contaminated food. The pork production sector has been hit hard by a series of L. monocytogenes-related food poisoning outbreaks in France. An overview of the diversity of strains circulating at all levels of the pork production chain, from pig farming to finished food products, is needed to identify the contamination routes and improve food safety. Until now, no typing data has been available on strains isolated across the entire pig and pork production chain. 
Here, we analyzed the population genetic structure of 687 L. monocytogenes strains isolated over the last 20 years in virtually all the French départements from three compartments of this production sector: pig farming (PF), the food processing environment (FPE) and finished food products (FFP). The genetic structure was described based on MLST clonal complexes (CCs). The CCs were obtained by mapping the PFGE profiles of the strains. The distribution of CCs was compared firstly between the three compartments and then with CCs obtained from 1106 strains isolated from other food production sectors in France. 
The predominant CCs of pig and pork strains were not equally distributed among the three compartments: the CC37, CC59 and CC77 strains, rarely found in FPE and FFP, were prevalent in PF. The two most prevalent CCs in the FPE and FFP compartments, CC9 and CC121, were rarely or never detected in PF. No CC was exclusively associated with the pork sector. Three CCs (CC5, CC6, CC2) were considered ubiquitous, because they were observed in comparable proportions in all food production sectors. The two most prevalent CCs in all sectors were CC9 and CC121, but their distribution was disparate. CC9 was associated with meat products and food products combining several food categories, whereas CC121 was not associated with any given sector. Based on these results, CC121 is likely able to colonize a larger diversity of food products than CC9. Both CCs being associated with the food production suggests, that certain processing steps, such as slaughtering or stabilization treatments, favor their settlement and the recontamination of the food produced.

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2018

Population genetic structure of Listeria monocytogenes strains isolated from the pig and pork meat production chain in France

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Benjamin Félix et al., Congrès international, IAFP, 25-27 avril 2018, Stockholm, Suède, poster

Listeria monocytogenes pathogenic bacterium, transmissible through contaminated food consumption. The pork meat sector has been hit hard by L. monocytogenes-related outbreaks in France. Numerous studies have been carried out to investigate the genetic diversity of L. monocytogenes pork strains from food or some food factories but never from the entire production chain.

Document réservé Espace Pro, veuillez vous identifier
2018