La base documentaire de l'IFIP

La base documentaire de l'IFIP : des centaines de documents à télécharger ou bien à commander.

Résultats 1 à 12 de 12 résultats
Rechercher une documentation
Publication Annéetrier par ordre croissant

PRRS post-vaccinal immune response to MLV in PRRS multivaccinaled sows

Consulter le resumé

Christelle Fablet (Anses) et al., 11th European Symposium of Porcine Management (ESPHM), 22-24 mai 2019, Utrecht, Pays-Bas

PRRS MLV vaccines are used to prevent reproductive disorders due to PRRSV in sows. Vaccine efficacy was proven only in sows vaccinated once or twice. In vaccinated herds, sows receive multiple vaccine boosters. To date, no data are available on the maintenance of PRRS immunity during these multiple revaccinations.

Document réservé Espace Pro, veuillez vous identifier
2019

Integrating blood transcriptome and immunity traits to identify markers of immune capacity in pigs

Consulter le resumé

T. Maroilley et al., 69th Annual meeting of the european federation of animal science (EAAP), Dubrovnik, Croatie, 27-31 août 2018

Understanding individual variability of immune capacity in livestock has become a priority to improve sustainability, with the aim to increase disease resistance and resilience in breeding programs. In this study, 550 60-day-old French Large White pigs vaccinated against Mycoplasma hyopneumoniae (M hyo) were monitored for 55 immunity traits (ITs) measured from blood samples (SUS_FLORA ANR funded project). The ITs included two types of parameters. First, parameters directly measured from blood: complete blood counts, counts of various cell subsets by flow cytometry, serum dosage of anti-M hyo IgG and haptoblobin. Second, parameters measured after in vitro stimulation of total blood: phagocytosis, production of cytokines (IL-1&”6;, IL-8, IL-10, IL-17, TNF&”5;, IFN&”7;) after stimulation by LPS or mitogenic agents. All animals were genotyped with 60K Illumina SNP chips. A subset of 243 piglets was chosen for blood transcriptome analysis using Agilent microarrays. We explored covariations between blood expression profiles and IT levels, and could draw lists of the most correlated genes with each IT. Each list represented candidate blood biomarkers potentially predictive of IT variations. As an example, we found 134 genes associated with phagocytosis capacity and we identified a subset of genes that could significantly predict levels of eight ITs related to phagocytosis by a sPLS approach. This gene subset included CXCR1, CCR1 and TLR2. Few candidate biomarkers were previously shown to be genetically controlled for their transcription in blood by eGWAS. Thus, our results provide new data to decipher the genetic architecture of IT variations. A next step will be to understand how IT variations could reflect individual robustness while facing pathogens, and how blood biomarkers could be used as predictors of immune capacity.

Document réservé Espace Pro, veuillez vous identifier
2018

Immunome differences between porcine ileal and jejunal Peyer’s patches revealed by global transcriptome sequencing of gut-associated lymphoid tissues

Consulter le resumé

T. Maroilley et al., Scientific Reports, 2018, volume 8, n° 1, 13 juin, 12 pages

The epithelium of the intestinal mucosa and the gut-associated lymphoid tissues (GALT) constitute an essential physical and immunological barrier against pathogens. In order to study the specificities of the GALT transcriptome in pigs, we compared the transcriptome profiles of jejunal and ileal Peyer’s patches (PPs), mesenteric lymph nodes (MLNs) and peripheral blood (PB) of four male piglets by RNA-Seq. We identified 1,103 differentially expressed (DE) genes between ileal PPs (IPPs) and jejunal PPs (JPPs), and six times more DE genes between PPs and MLNs. The master regulator genes FOXP3GATA3STAT4TBX21 and RORC were less expressed in IPPs compared to JPPs, whereas the transcription factor BCL6 was found more expressed in IPPs. In comparison between IPPs and JPPs, our analyses revealed predominant differential expression related to the differentiation of T cells into Th1, Th2, Th17 and iTreg in JPPs. Our results were consistent with previous reports regarding a higher T/B cells ratio in JPPs compared to IPPs. We found antisense transcription for respectively 24%, 22% and 14% of the transcripts detected in MLNs, PPs and PB, and significant positive correlations between PB and GALT transcriptomes. Allele-specific expression analyses revealed both shared and tissue-specific cis-genetic control of gene expression.

https://www.nature.com/articles/s41598-018-27019-7.pdf

2018

Deciphering the genetic regulation of peripheral blood transcriptome in pigs through expression genome-wide association study and allele-specific expression analysis

Consulter le resumé

T. Maroilley et al., BMC Genomics, 2017, 13 décembre, volume 18, n° 1, 13 décembre,19 pages

Abstract

BACKGROUND:

Efforts to improve sustainability in livestock production systems have focused on two objectives: investigating the genetic control of immune function as it pertains to robustness and disease resistance, and finding predictive markers for use in breeding programs. In this context, the peripheral blood transcriptome represents an important source of biological information about an individual's health and immunological status, and has been proposed for use as an intermediate phenotype to measure immune capacity. The objective of this work was to study the genetic architecture of variation in gene expression in the blood of healthy young pigs using two approaches: an expression genome-wide association study (eGWAS) and allele-specific expression (ASE) analysis.

RESULTS:

The blood transcriptomes of 60-day-old Large White pigs were analyzed by expression microarrays for eGWAS (242 animals) and by RNA-Seq for ASE analysis (38 animals). Using eGWAS, the expression levels of 1901 genes were found to be associated with expression quantitative trait loci (eQTLs). We recovered 2839 local and 1752 distant associations (Single Nucleotide Polymorphism or SNP located less or more than 1 Mb from expression probe, respectively). ASE analyses confirmed the extensive cis-regulation of gene transcription in blood, and revealed allelic imbalance in 2286 SNPs, which affected 763 genes. eQTLs and ASE-genes were widely distributed on all chromosomes. By analyzing mutually overlapping eGWAS results, we were able to describe putative regulatory networks, which were further refined using ASE data. At the functional level, genes with genetically controlled expression that were detected by eGWAS and/or ASE analyses were significantly enriched in biological processes related to RNA processing and immune function. Indeed, numerous distant and local regulatory relationships were detected within the major histocompatibility complex region on chromosome 7, revealing ASE for most class I and II genes.

CONCLUSIONS:

This study represents, to the best of our knowledge, the first genome-wide map of the genetic control of gene expression in porcine peripheral blood. These results represent an interesting resource for the identification of genetic markers and blood biomarkers associated with variations in immunity traits in pigs, as well as any other complex traits for which blood is an appropriate surrogate tissue.

2017

Deciphering the genetic control of innate and adaptive immune responses in pig: a combined genetic and genomic study

Consulter le resumé

Improving animal robustness and resistance to pathogens by adding health criteria in selection schemes is one of the challenging objectives of the next decade. In order to better understand the genetic control of immunity in French Large White pigs, we have launched a program combining genetic and genomic studies not focussing on any particular pathogen.
PDF icon Deciphering the genetic control of innate and adaptive immune responses in pig: a combined genetic and genomic study
2011

Décryptage du contrôle génétique des réponses immunitaires innées et adaptatives chez le porc Large White : une étude combinant des approches génétiques et fonctionnelles

Consulter le resumé

Un programme d'analyse fine du contrôle génétique de la réponse immunitaire (RI) combinant des approches génétiques et fonctionnelles a été développé. Plus de 400 animaux de race Large White mesurés pour des caractères de production ont été caractérisés pour un large éventail de paramètres de l'immunité trois semaines après vaccination contre Mycoplasma hyopneumoniae.
PDF icon Décryptage du contrôle génétique des réponses immunitaires innées et adaptatives chez le porc Large White : une étude combinant des approches génétiques et fonctionnelles
2011

Deciphering the genetic control of innate and adaptive immune responses in pig: a combined genetic and genomic study

Consulter le resumé

Improving animal robustness and resistance to pathogens by adding health criteria in selection schemes is one of the challenging objectives of the next decade. In order to better understand the genetic control of immunity in French Large White pigs, we have launched a program combining genetic and genomic studies not focussing on any particular pathogen.
2010

A genetic and functional analysis of innate and adaptive immunity in pigs

Consulter le resumé

PDF icon A genetic and functional analysis of innate and adaptive immunity in pigs
2010

Genetic analysis of the immune response in pigs

Consulter le resumé

Resistance to disease and immunocompetence in livestock production is a complex issue. Introducing health criteria in the next generation selection schemes is one of the challenging objectives of the next decade. In this context, our aim is to study the genetic determinism of immune response in pig without focussing on a particular pathogen. A population of 443 60-day old castrated Large White males recorded for production traits in a testing farm unit was scored for various immunity parameters three weeks after vaccination against Mycoplasma Hyopneumoniae.
2010

Etude de la réponse immunitaire des porcs français de race Large White et analyse conjointe du transcriptome des leucocytes du sang périphérique

Consulter le resumé

Depuis plus de 25 ans maintenant, un programme de sélection génétique ciblant des caractères de production a été poursuivi en France dans l’espèce porcine. Associé à l’application de mesures sanitaires et médicales strictes, il a permis une nette amélioration des performances zootechniques des animaux. Cependant, la législation européenne, de plus en plus préoccupée par le bien-être animal et la sécurité du consommateur, a récemment interdit l’utilisation d’antibiotiques comme facteurs régulateurs de croissance et tend à réduire de manière générale les mesures prophylactiques médicamenteuses.
PDF icon Etude de la réponse immunitaire des porcs français de race Large White et analyse conjointe du transcriptome des leucocytes du sang périphérique
2009

Etude de la réponse immunitaire des porcs français de race Large White et analyse conjointe du transcriptome des leucocytes du sang périphérique

Consulter le resumé

L’espèce porcine a bénéficié depuis 20 ans d’un programme de sélection génétique qui a ciblé des caractères de production et cette sélection, accompagnée du respect de règles sanitaires strictes incluant vaccination et antibiothérapie préventive, a permis une nette amélioration des performances zootechniques. Or, dans une perspective de respect accru du bien-être animal et de la sécurité du consommateur, les normes européennes visent une réduction des mesures prophylactiques médicamenteuses.
PDF icon Etude de la réponse immunitaire des porcs français de race Large White et analyse conjointe du transcriptome des leucocytes du sang périphérique
2008

Influence de deux probiotiques sur l'immunité du porcelet

Consulter le resumé

L’influence de 2 probiotiques, Pediococcus acidilactici et la levure Saccharomyces cerevisiae boulardii, sur la réponse immunitaire a été étudiée. Dès la naissance, des portées de porcelets ont reçu 5 traitements 1) témoin sans antibiotique ou 2) avec antibiotique (tiamuline), 3) Pediococcus a., 4) Saccharomyces c. et 5) les 2 probiotiques. Parmi les sevrés, certains ont subi une infection avec E. Coli.
PDF icon Influence de deux probiotiques sur l'immunité du porcelet
2005