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Ecology of Salmonella and antimicrobial resistance in a pig slaughterbouse

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Arnaud Bridier (Anses) et al., 13e SafePork, 26-29 août 2019, Berlin, Allemagne

Salmonella: a public health issue
• 1st pathogen in terms of deaths related to contaminated food in France
• > 90 000 salmonellosis per year in Europe
• Multi resistance to antibiotics in the food chain
• Role of cleaning and disinfection (C&D) procedures in the selection of antibiotics resistant bacteria ?

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2019

A Salmonella database to monitor and centralize regulatory own-checks results (CE) n° 2073/2005 obtained by slaugterhouse

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Sabine Itié-Hafez (Ministère de l'Agriculture) et Alain Le Roux (Ifip), 13e SafePork, 26-29 août 2019, Berlin, Allemagne, poster

Salmonellosis is a major cause of foodborne outbreaks caused by bacteria in Europe. In 2014, the European Commission reinforced the survey of this contaminant in the pig and pork industry by the competent authority. In this context, French General Directorate for Food required a new system to centralize regulatory own-checks results for Salmonella in pig carcasses.

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2019

Impact of cleaning and disinfection procedures on microbial ecology and Salmonella antimicrobial resistance in a pig slaughterhouse

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Arnaud Bridier (Anses) et al., Scientific Reports, 2019, volume 9, n° 1, 10 septembre, 13 pages

To guarantee food safety, a better deciphering of ecology and adaptation strategies of bacterial pathogens such as Salmonella in food environments is crucial. The role of food processing conditions such as cleaning and disinfection procedures on antimicrobial resistance emergence should especially be investigated. In this work, the prevalence and antimicrobial resistance of Salmonella and the microbial ecology of associated surfaces communities were investigated in a pig slaughterhouse before and after cleaning and disinfection procedures. Salmonella were detected in 67% of samples and isolates characterization revealed the presence of 15 PFGE-patterns belonging to five serotypes: S.4,5,12:i:-, Rissen, Typhimurium, Infantis and Derby. Resistance to ampicillin, sulfamethoxazole, tetracycline and/or chloramphenicol was detected depending on serotypes. 16S rRNA-based bacterial diversity analyses showed that Salmonella surface associated communities were highly dominated by the Moraxellaceae family with a clear site-specific composition suggesting a persistent colonization of the pig slaughterhouse. Cleaning and disinfection procedures did not lead to a modification of Salmonella susceptibility to antimicrobials in this short-term study but they tended to significantly reduce bacterial diversity and favored some genera such as Rothia and Psychrobacter. Such data participate to the construction of a comprehensive view of Salmonella ecology and antimicrobial resistance emergence in food environments in relation with cleaning and disinfection procedures.

https://www.nature.com/articles/s41598-019-49464-8.pdf

2019

Influence of reduced levels or suppression of sodium nitrite on the growth of psychrotrophic Clostridium botulinum group II type B in cooked ham and frankfurters

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Sarah Lebrun (Université  de Liège) et al., 65th International Congress of Meat Science and Technology (ICOMST), 4-9 août 2019, Potsdam/Berlin, Allemagne, poster, p. 28

Sodium nitrite (NaNO2) is commonly used in meat products manufacturing for its antimicrobial effect on Clostridium botulinum, responsible of botulism (a deadly paralytic disease) by production of neurotoxins. Its use can nevertheless be associated with nitrosamines production, which are considered as carcinogenic substances (EFSA, The EFSA Journal, 2013, 14: 1-31). The objective of the present study was to compare the growth and toxinogenesis of psychrotrophic C. botulinum Group II (non-proteolytic) type B in cooked ham and in frankfurters in function of the NaNO2 incorporation rate (0, 30, 60 and 80 mg/kg) in order to evaluate the risk associated with this pathogen in two common cooked meat products.

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2019

Services aux entreprises à partir de la base de données des autocontrôles réalisés en abattage-découpe

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Alain Le Roux, bilan 2018, éditions IFIP, avril 2019, p. 52

En 2017, l’IFIP a développé dans le site internet PDC un module qui permet aux entreprises d’abattage-découpe de porcs de saisir les résultats de leurs contrôles concernant les contaminants chimiques (métaux lourds, radionucléides, les résidus médicamenteux), les contaminants microbiologiques et les dangers physiques (corps étrangers).
En 2018, l’interface de PDC a été ouverte aux ruminants.
PDF icon Alain Le Roux, bilan 2018, éditions IFIP, avril 2019, p. 52
2019

Nouvelle version du Guide de Bonnes Pratiques d'Hygiène pour l'abattage et la découpe de porc

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Arnaud Bozec, bilan 2018, éditions IFIP, avril 2019, p. 50

L’IFIP en partenariat avec les organisations professionnelles d’abattage et de découpe, ont révisé le GBPH abattage découpe de porcs de manière à ce que les professionnels du secteur disposent d’un guide validé par l’Administration française.

PDF icon Arnaud Bozec, bilan 2018, éditions IFIP, avril 2019, p. 50
2019

Evaluation de l’efficacité de thermomètres pour la mesure de la température de surface des carcasses

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Alain Le Roux, bilan 2018, éditions IFIP, avril 2019, p. 51

C’est en se basant sur les avis de l’EFSA (European Food Safety Autority) et de l’ANSES (Agence Nationale de Sécurité Sanitaire) que le Règlement (UE) 2017/1981 a été publié le 31 octobre 2017 (JOCE du 1er novembre 2017). Ce règlement modifie l’annexe III du règlement (CE) n°853/2004 concernant les conditions de température pendant le transport de la viande, en introduisant un nouveau critère basé sur la température de surface des carcasses. Désormais, pour transporter les carcasses, les industriels peuvent, par dérogation, déroger au critère des 7°C maximum en tout point.
Le but de l’étude demandée par les industriels de la viande est de caractériser la mesure de température de surface des carcasses de viande en comparant les technologies existantes.
Dans ce but, des tests sur des matrices des espèces bovine et porcine, sur plusieurs carcasses et à différentes températures, ont été réalisés. Nous avons effectué ces tests avec 3 types de thermomètres : de contacts, infrarouges et de pénétration.
PDF icon Alain Le Roux, bilan 2018, éditions IFIP, avril 2019, p. 51
2019

Evaluation d’un outil moléculaire pour valider les mesures de maîtrise des salmonelles dans la filière

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Sabine Jeuge, bilan 2018, éditions IFIP, avril 2019, p. 45

En 2017, Salmonella spp. a été responsable de 30 % des toxi-infections alimentaires collectives selon Santé Publique France.
Peu de données quantitatives sont disponibles aux différents maillons de la filière porcine. La méthode de référence (ISO/TS 6579-2:2012) pour le dénombrement de Salmonella spp. présente l’inconvénient d’être chronophage avec un délai de 5 jours avant obtention du résultats de dénombrement. Les méthodes alternatives sur milieux chromogènes sont utilisables sur certaines matrices pauvres en flore annexe et ne permettent pas une récupération optimale des bactéries présentes mais stressées. Or, le long de la chaîne de l’élevage à la transformation de produit en passant par l’abattage, les bactéries subissent des nombreux stress entrainant des changements physiologiques, et un passage à l’état de bactéries viables mais non cultivables (VNC).
L’objectif de ce projet est, dans un premier temps, la mise au point d’une méthode de dénombrement des salmonelles pour les échantillons d’élevage (fèces) et les carcasses de porc basée sur une PCR TaqMan (qPCR) et avec un traitement par un marqueur de viabilité, le propidium monoazide (PMA) afin d’éliminer l’amplification d’ADN des bactéries mortes des matrices

PDF icon Sabine Jeuge, bilan 2018, éditions IFIP, avril 2019, p. 45
2019

Diversité génétique des souches de Listeria isolées dans la filière porcine en France

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Carole Feurer, bilan 2018, éditions IFIP, avril 2019, p. 46

Listeria monocytogenes (Lm) est une bactérie responsable d’une zoonose rare mais grave appelée listériose qui cause 300 à 400 infections par an en France et s’avère mortelle dans 20 à 30% des cas. Elle touche principalement les personnes immunitairement affaiblies. La gravité de l’infection dépend de la dose et de la virulence du groupe génétique de la souche ingérée.
La contamination des aliments peut survenir à partir de matières premières animales ou végétales mais plus particulièrement à partir de l’environnement des sites de production dans lequel les souches de Lm sont capables de survivre, de persister et de s’implanter. La technique MLST (Multi Locus Sequence Typing) est devenue la méthode standardisée au niveau international pour analyser la structure génétique des populations de Lm. Fondée sur le polymorphisme de séquence de 7 gènes de ménage, cette méthode permet l’attribution d’un «complexe clonal» (CC) à une souche. En France, les CC1, CC2, CC4, CC6 sont décrits comme hyper virulents et fréquemment isolés de cas cliniques.
Les CC9 et CC121 sont quant à eux isolés plus fréquemment d’aliments que de cas cliniques. Les CC9 et CC121 sont prévalents dans tous les compartiments de production alimentaire.
Entre 2015 et 2017, l’Ifip en partenariat avec l’Anses de Maisons-Alfort ont mené une étude qui visait à obtenir une meilleure connaissance de la diversité des souches de Listeria monocytogenes (Lm) afin de mieux caractériser la manière dont elle circule dans la filière porcine.
PDF icon Carole Feurer, bilan 2018, éditions IFIP, avril 2019, p. 46
2019

Dispositif de maîtrise des salmonelles dans la filière porcine française

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Gilles Nassy, bilan 2018, éditions IFIP, avril 2019, p. 44

Les actions de la filière porcine française pour maîtriser le risque de salmonelle sont nombreuses et réparties sur chaque maillon de notre filière. Il n’est pas simple de connaître l’ensemble de ces mesures et d’avoir une idée de la cohérence du dispositif quand on se trouve au sein d’un maillon.
L’Ifip qui bénéficie d’une vue d’ensemble a décrit dans ce programme l’ensemble du dispositif de maîtrise, afin de souligner sa cohérence et sa pertinence. La DGAL et l’ANSES ont également participé à ce travail descriptif.
Outre le partage de la connaissance du dispositif salmonelle français pour tous les opérateurs de la filière, ce travail a également vocation à aider les entreprises exportatrices à expliquer aux services sanitaires étrangers l’architecture et la performance du dispositif français.
Ainsi la description est technique mais aussi pédagogique pour les techniciens comme les commerciaux oeuvrant sur les marchés exports de viandes et de charcuteries.

PDF icon Gilles Nassy, bilan 2018, éditions IFIP, avril 2019, p. 44
2019

Évaluation d’une méthode de quantification des bactéries viables de Listeria après désinfection

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Bastien Frémaux, bilan 2018, éditions IFIP, avril 2019, p. 47

Le nettoyage et la désinfection (N&D) des installations en agroalimentaire est une procédure fondamentale pour garantir la qualité et la sécurité sanitaire des aliments. La validation de l’efficacité des opérations de N&D repose le plus souvent sur un contrôle visuel de l’état de propreté des surfaces et sur la mise en oeuvre d’analyses microbiologiques classiques basées sur des méthodes culturales.
Il est aujourd’hui connu que certains stress, tels que l’exposition aux désinfectants, peuvent conduire à l’apparition de bactéries viables non cultivables (VNC), non détectées dans les procédures de contrôle actuelles. Leur présence non contrôlée sur les surfaces d’ateliers peut être problématique en cas de transfert à l’aliment en contact, où elles pourront se revivifier et se multiplier.
Ce programme visait (1) au développement et à la mise en oeuvre au laboratoire d’une méthode de PMA-PCRq pour la quantification des formes viables de L. monocytogenes, (2) à évaluer la PMAPCRq pour la quantification des formes viables (dont les non cultivables) de L.
monocytogenes
adhérentes en biofilm sur deux types de surface (acier inoxydable et PET) suite à l’exposition à 3 produits désinfectants largement employés dans l’industrie charcutière et plus généralement dans l’agroalimentaire : un alcalin chloré, une formulation à base d’ammonium quaternaire et de glutaraldéhyde et une formulation à base d’acide peracétique.
PDF icon Bastien Frémaux, bilan 2018, éditions IFIP, avril 2019, p. 47
2019

Complete genome sequence of Salmonella enterica subsp. enterica Serotype Derby, associated with the pork sector in France

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Yann Sévellec et al., Microbiology Resource Announcements, volume 7, n° 12, septembre, 4 pages

In the European Union, Salmonella enterica subsp. enterica serovar Derby is the most abundant serotype isolated from pork. Recent studies have shown that this serotype is polyphyletic. However, one main genomic lineage, characterized by sequence type 40 (ST40), the presence of the Salmonella pathogenicity island 23, and showing resistance to streptomycin, sulphonamides, and tetracycline (STR-SSS-TET), is pork associated. Here, we describe the complete genome sequence of a strain from this lineage isolated in France.

https://mra.asm.org/content/ga/7/12/e01027-18.full-text.pdf

2018

Redlosses: reducing food losses by microbial spoilage prediction

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M. Zagorec (INRA) et al., EFFOST, 6-8 novembre 2018, Nantes

Food spoilage leads to significant wastes and losses, and is an important economic issue in food industry. In the case of meat, a large part of spoilage is the consequence of bacterial growth and subsequent metabolic activities causing organoleptic spoilage of the final product (defects in texture, color, odor, or aspect), leading finally to products that are lost because they do not fit the quality standards. In addition, meat production chain requires energy, water and cost consuming operations (i.e. animal breeding, slaughtering, and transformation and storage which are usually performed at low temperature). Therefore meat product spoilage that appears at the end of the process or during shelf life affects the whole production chain performances as well as the sustainability label of the meat sector. The objective of the project is to reduce food losses by predicting, early in the production process, the onset of bacterial spoilage during storage in order to propose decision-support tools for directing process. Pork and poultry meat, the two main meats consumed in France will be studied. The economic impact of losses of these products will be assessed. Dynamics of bacterial communities will be monitored during processing steps (from primary cuts to end products at use-by-date and beyond) and various descriptors of spoilage will be measured. The natural variability between batches and that associated with production processes will be considered. Data will be used to identify accurate spoilage markers and to compute innovative mathematical models for predicting spoilage occurrence as a function of the initial composition of the microbiota (diversity and abundance) and some abiotic factors (lactate concentration, nature of packaging atmosphere). The models will be validated on meat products, including the economic aspect in order to propose decision-support tools for the food producers. The project and first results will be presented.

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2018

Plus de flexibilité pour les transformateurs à la ferme

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Arnaud Bozec, Réussir Porc  - Tech Porc (FRA), 2018, n°263, novembre, p.14

La réglementation sanitaire pour les ateliers de transformation à la ferme va évoluer fin 2018...

PDF icon Arnaud Bozec, Réussir Porc-Tech Porc (FRA), 2018, n°263, novembre, p.14
2018

One Health et sécurité sanitaire : de la plante à l'animal consommateur et consommé

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Visuels de Eric Royer, Séminaire Réseau Français Santé Végétale, 3 octobre 2018, Paris, 44 pages

PDF icon Visuels de Eric Royer, Séminaire Réseau Français Santé Végétale, 3 octobre 2018, Paris, 44 pages
2018

Ecology of Salmonella and antimicrobial resistance in a pig slaughterhouse

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Arnaud Bridier et al., Food Micro, 3-6 septembre 2018, Berlin, Allemagne et I3S, 24-26 septembre 2018, Saint-Malo, France

Salmonella is a bacterial pathogen responsible for a large number of food associated infections. In order to guarantee food safety, a better understanding of Salmonella ecology and adaptation strategies on the food production chain constitutes a prerequisite. In a One Health perspective, data on Salmonella antibiotic resistance in food environments are also crucial to decipher transmission routes of resistant foodborne pathogens as well as resistance genetic determinants involved, and the role of process and selection pressures underwent in food industries (as cleaning and disinfection) in bacterial adaptation and antimicrobial resistance emergence.
Using a pig slaughterhouse as a model food environment, occurrence of Salmonella was investigated at six different areas along the slaughter chain and through 4 sampling campaign, each time before and after cleaning and disinfection procedures (a total of 48 surface samples). Characterization of isolated Salmonella strains using serotyping and pulsotyping enabled to identify and trace persistent strains in the slaughterhouse. Minimal inhibiting concentrations (MIC) were also determined for various relevant antibiotics and for biocides used in the slaughterhouse. In addition, associated indigenous bacterial communities were characterized using 16S rRNA amplicon sequencing. 
Results revealed the presence of Salmonella at all sampling area. Five serotypes were identified: S.4,5,12:i:- (50%), Rissen (16%), Typhimurium (16%), Infantis (10%) and Derby (8%). Strains were found at different dates and potentially at the same sampling area suggesting they persist in the slaughterhouse despite of the cleaning and disinfection procedures. Approximately 70% of isolated Salmonella strains exhibit resistance to ampicillin and sulfamethoxazole, 80% to tetracycline and 10% to chloramphenicol. There was no evolution of CMI comparing strains before and after cleaning and disinfection procedures concerning both biocides and antibiotics. Bacterial diversity analyses showed that populations in slaughterhouse were highly dominated by &a1;-proteobacteria and especially by the Moraxellaceae family (genus Psychrobacter, Moraxella, Enhydrobacter and Acinetobacter) at the different sampling areas. Population compositions were overall stable in time at a given sampling area suggesting that the surface populations are resident populations within the slaughterhouse, rather than populations introduced each week by the new swine bands. Cleaning and disinfection procedures tend to reduce bacterial diversity by eliminating the minority species but did not greatly impact the composition of bacterial communities with regards to the dominant species.
Together, such data participate to the construction of a comprehensive view of Salmonella ecology in food environments integrating associated resident microbial flora and the distribution of antimicrobial resistance in relation to processing conditions.

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2018

Population genetic structure of Listeria monocytogenes strains isolated from the pig and pork meat production chain in France

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Benjamin Félix (Anses) et al., Food Micro, 3-6 septembre 2018, Berlin, Allemagne

Listeria monocytogenes is an ubiquitous pathogenic bacterium, transmissible to humans through the consumption of contaminated food. The pork production sector has been hit hard by a series of L. monocytogenes-related food poisoning outbreaks in France. An overview of the diversity of strains circulating at all levels of the pork production chain, from pig farming to finished food products, is needed to identify the contamination routes and improve food safety. Until now, no typing data has been available on strains isolated across the entire pig and pork production chain. 
Here, we analyzed the population genetic structure of 687 L. monocytogenes strains isolated over the last 20 years in virtually all the French départements from three compartments of this production sector: pig farming (PF), the food processing environment (FPE) and finished food products (FFP). The genetic structure was described based on MLST clonal complexes (CCs). The CCs were obtained by mapping the PFGE profiles of the strains. The distribution of CCs was compared firstly between the three compartments and then with CCs obtained from 1106 strains isolated from other food production sectors in France. 
The predominant CCs of pig and pork strains were not equally distributed among the three compartments: the CC37, CC59 and CC77 strains, rarely found in FPE and FFP, were prevalent in PF. The two most prevalent CCs in the FPE and FFP compartments, CC9 and CC121, were rarely or never detected in PF. No CC was exclusively associated with the pork sector. Three CCs (CC5, CC6, CC2) were considered ubiquitous, because they were observed in comparable proportions in all food production sectors. The two most prevalent CCs in all sectors were CC9 and CC121, but their distribution was disparate. CC9 was associated with meat products and food products combining several food categories, whereas CC121 was not associated with any given sector. Based on these results, CC121 is likely able to colonize a larger diversity of food products than CC9. Both CCs being associated with the food production suggests, that certain processing steps, such as slaughtering or stabilization treatments, favor their settlement and the recontamination of the food produced.

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2018

Population Genetic Structure of Listeria monocytogenes Strains Isolated From the Pig and Pork Production Chain in France

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Benjamin Félix (Anses) et al., Frontiers in Microbiology, 2018, n° 9, 6 avril, 11 pages

Listeria monocytogenes is an ubiquitous pathogenic bacterium, transmissible to humans through the consumption of contaminated food. The pork production sector has been hit hard by a series of L. monocytogenes-related food poisoning outbreaks in France. An overview of the diversity of strains circulating at all levels of the pork production chain, from pig farming (PF) to finished food products (FFP), is needed to identify the contamination routes and improve food safety. Until now, no typing data has been available on strains isolated across the entire pig and pork production chain. Here, we analyzed the population genetic structure of 687 L. monocytogenes strains isolated over the last 20 years in virtually all the French départements from three compartments of this production sector: PF, the food processing environment (FPE), and FFP. The genetic structure was described based on Multilocus sequence typing (MLST) clonal complexes (CCs). The CCs were obtained by mapping the PFGE profiles of the strains. The distribution of CCs was compared firstly between the three compartments and then with CCs obtained from 1106 strains isolated from other food production sectors in France. The predominant CCs of pig and pork strains were not equally distributed among the three compartments: the CC37, CC59, and CC77 strains, rarely found in FPE and FFP, were prevalent in PF. The two most prevalent CCs in the FPE and FFP compartments, CC9 and CC121, were rarely or never detected in PF. No CC was exclusively associated with the pork sector. Three CCs (CC5, CC6, and CC2) were considered ubiquitous, because they were observed in comparable proportions in all food production sectors. The two most prevalent CCs in all sectors were CC9 and CC121, but their distribution was disparate. CC9 was associated with meat products and food products combining several food categories, whereas CC121 was not associated with any given sector. Based on these results, CC121 is likely able to colonize a larger diversity of food products than CC9. Both CCs being associated with the food production suggests, that certain processing steps, such as slaughtering or stabilization treatments, favor their settlement and the recontamination of the food produced. 

https://www.frontiersin.org/articles/10.3389/fmicb.2018.00684/pdf

2018

Selection procedure of bioprotective cultures for their combined use with High Pressure Processing to control spore-forming bacteria in cooked ham.

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Mihanta Ramaroson et al., International journal of food microbiology, 2018, voluime 276, 2 juillet, p. 28-38

High Pressure Processing (HPP) and biopreservation can contribute to food safety by inactivation of bacterial contaminants. However these treatments are inefficient against bacterial endospores. Moreover, HPP can induce spore germination. The objective of this study was to select lactic acid bacteria strains to be used as bioprotective cultures, to control vegetative cells of spore-forming bacteria in ham after application of HPP. A collection of 63 strains of various origins was screened for their antagonistic activity against spore-forming Bacillus and Clostridium species and their ability to resist to HPP. Some safety requirements should also be considered prior to their introduction into the food chain. Hence, the selection steps included the assessment of biogenic amine production and antibiotic resistance. No strain produced histamine above the threshold detection level of 50 ppm. From the assessment of antibiotic resistance against nine antibiotics, 14 susceptible strains were kept. Antagonistic action of the 14 strains was then assessed by the well diffusion method against pathogenic or spoilage spore-forming species as Bacillus cereusClostridium sp. like botulinum, Clostridium frigidicarnis, and Clostridium algidicarnis. One Lactobacillus curvatus strain and one Lactococcus lactis strain were ultimately selected for their widest inhibitory spectrum and their potential production of bacteriocin. A Lactobacillus plantarum strain was included as control. Their resistance to HPP and ability to regrow during chilled storage was then assessed in model ham liquid medium. Treatments of pressure intensities of 400, 500, and 600 MPa, and durations of 1, 3, 6, and 10 min were applied. After treatment, cultures were incubated at 8 °C during 30 days. Inactivation curves were then fitted by using a reparameterized Weibull model whereas growth curves were modelled with a logistic model. Although the two Lactobacillus strains were more resistant than L. lactis to HPP, the latter was the only strain able to regrow following HPP. The absence of biogenic amine production of this strain after growth on diced cube cooked ham was also shown. In conclusion this L. lactis strain could be selected as representing the best candidate for a promising preservative treatment combining biopreservation and HPP to control spore-forming bacteria in cooked ham.

 
2018

Validation de l’utilisation des éponges sur carcasses pour vérifi er le respect des critères d’hygiène des procédés et le pilotage de l’hygiène

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Alain Le Roux, bilan 2017, éditions IFIP, mai 2018, p. 85

De nombreuses études ont été publiées ces dernières décennies sur la comparaison des méthodes destructives et non destructives pour l’analyse de la contamination microbiologique de surface des carcasses. Le Règlement Européen 2073/2005 impose aux opérateurs de respecter les critères d’hygiène des procédés, définis pour les carcasses de bovins (BV), d’ovins (OV) et de porcins (PC) en termes de log moyen quotidien (LMQ) de nombre de colonies aérobies (FAM) et d’entérobactéries (ENT), calculé sur 5 carcasses prélevées le même jour. Bien que ces limites réglementaires
s’appliquent uniquement aux carcasses prélevées par excision, d’autres méthodes de prélèvement peuvent être utilisées s’il est démontré, à la satisfaction de l’autorité compétente, qu’elles fournissent des garanties au moins équivalentes. En France, depuis 20 ans, la méthode de prélèvement par excision est la méthode de référence. Les entreprises des filières viandes s’interrogent sur l’utilisation de cette méthode non destructive pour réaliser également les dénombrements de FAM et ENT. Cette étude a ainsi été conçue pour pouvoir évaluer sur carcasses de bovins, ovins et porcins, l’impact de la méthode par éponge sur le calcul des critères d’hygiène des procédés réglementaires, comparativement à la méthode de référence par excision.

PDF icon Alain Le Roux, bilan 2017, éditions IFIP, mai 2018, p. 85, fiche n° 47
2018

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