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Toward the prediction of the PSE-Like muscle mefect in cooked hams

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Laetitia Théron (Inrae), Meat and Muscle Biology, 2020, volume 4, n°2, juillet, 6 pages

Pale Soft Exudative (PSE)-like muscle defect is of great importance in the cooked ham industry because of the economic losses it can cause. The flagship product is the “Jambon supérieur,” a polyphosphate-free cooked ham, usually sold sliced and packaged. Slicing is an automatic process that reveals the defect as holes in the slice leading to slicing losses. Up to now, the PSE-like defect has only been detected on raw meat after deboning the pork leg because it affects the inner part of the semimembranosus muscles and also the adductor muscles. The objective of this study was to develop innovative approaches that combine mechanistic elucidation and the discovery of potential biomarkers (i) at the level of the muscle and (ii) at the level of the live animal by analyzing proteins from plasma. The use of chemometrics for the spectral fingerprinting of pig plasma was chosen to predict the PSE-like muscle defect in raw hams.

source : https://www.iastatedigitalpress.com/mmb/article/11156/galley/10837/download/

2020

Toward the prediction of PSE-like muscle defect in hams: Using chemometrics for the spectral fingerprinting of plasm

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Laetitia Théron (Inrae), Food Control, 2020, volume 109, mars

PSE-like muscle defect is of great importance in the cooked ham industry because of the economic losses it can cause. Although this muscle defect has been widely studied from a mechanistic angle, there is a lack of reliable and practical markers. This study proposes the use of chemometrics for the spectral fingerprinting of pig plasma to predict the PSE-like muscle defect in cooked hams. Both MALDI-TOF mass spectrometry and ATR-FTIR spectroscopy were used to obtain protein and chemical function fingerprints. Though the two approaches showed very good predictive capacity, MALDI-TOF mass spectrometry was found to be better suited to predicting normal hams, i.e. hams without the defect; while ATR-FTIR spectroscopy was found to be better suited to predicting the PSE-like muscle defect. These results demonstrated the feasibility of predicting the PSE-like defect using spectral fingerprinting and chemometrics approaches.

2020

Urinary signature of pig carcasses with boar taint by liquid chromagraphy-high resolution mass spectrometry

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Cristina C. Jacob et al., Food Additives & Contaminants : Part A, version pas encore finalisée, 29 pages

Boar taint is an offensive odor that can occur while cooking pork or pork products and is identified in some uncastrated male pigs that have reached puberty. It is widely held that boar taint is the result of the accumulation in back-fat of two malodorous compounds: androstenone and skatole. The purpose of the present study was to assess a mass spectrometry-based metabolomics strategy to investigate the metabolic profile of urine samples from pig carcasses presenting low (untainted) and high (tainted) levels of androstenone and skatole in back fat. Urine samples were analyzed by LC-ESI(+)-HRMS. Discrimination between tainted and untainted animals was observed by application of multivariate statistical analysis, which allowed to highlight candidate urinary biomarkers. These urinary metabolites were positively correlated to androstenone and skatole levels in back fat. Therefore, the present study suggested that the measurement of these urinary metabolites might provide information with regard to androstenone and skatole levels in live pigs.

2017